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在淡色库蚊越冬过程中,测出其血淋巴含18～19种游离氨基酸。越冬期淡色库蚊除胱氨酸和脯氨酸浓度(0.2067±0.0058nmol/μl、36.5467±0.4654nmol/μl)高于越冬前期(0.1563±0.0021nmol/μl、22.2467±1.3537nmol/μl)外,其余各氨基酸及总氨基酸均显著下降;越冬后期与越冬期相比,各氨基酸及总氨基酸均显著升高,其中天门冬氨酸(0.4300±0.0520nmol/μl)、谷氨酸(3.9600±0.0794nmol/μl)、丙氨酸(22.9067±1.2860nmol/μl)、胱氨酸(1.0167±0.0551nmol/μl)和脯氨酸(47.9267±2.5597nmol/μl)及总氨基酸(98.2402±3.6139nmol/μl)甚至超过越冬前期的水平(分别为0.1900±0.0557、3.3067±0.1850、6.8833±0.1250、0.1563±0.0021、22.2467±1.3537和59.9617±1.5296nmol/μl)。用于采集血淋巴的蚊虫,胃血均为Sella 1期,卵巢发育不超过Christophers IIb期,可排除血食及卵巢后期发育对氨基酸测定结果的影响。
During the overwintering process of Culex pipiens pallens, 18 to 19 kinds of free amino acids were detected in hemolymph. Overwintering period, Culex pipiens pallens were higher than cytoplasmic and proline concentrations (0.2067 ± 0.0058nmol / μl, 36.5467 ± 0.4654nmol / μl) than pre-winter (0.1563 ± 0.0021nmol / μl, 22.2467 ± 1.3537nmol / μl) While the other amino acids and total amino acids decreased significantly. Compared with the overwintering period, the amino acids and total amino acids were significantly increased in the late winter, including aspartate (0.4300 ± 0.0520nmol / μl), glutamate (3.9600 ± 0.0794nmol / μl), alanine (22.9067 ± 1.2860nmol / μl), cystine (1.0167 ± 0.0551nmol / μl) and proline (47.9267 ± 2.5597nmol / μl) and total amino acids (98.2402 ± 3.6139nmol / μl) Even exceeding pre-winter levels (0.1900 ± 0.0557, 3.3067 ± 0.1850, 6.8833 ± 0.1250, 0.1563 ± 0.0021, 22.2467 ± 1.3537 and 59.9617 ± 1.5296 nmol / μl respectively). Used to collect hemolymph mosquitoes, stomach blood are Sella 1, ovarian development does not exceed Christophers IIb period, can exclude blood and ovarian development of amino acid determination results.