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目的：观察不同强度高功率微波(HPM)对小鼠免疫组织和外周血淋巴细胞的影响及其作用机制。方法：应用TUNEL和FCM(Annexin—V/PI双标法)技术研究小鼠胸腺、脾脏、淋巴结和外周血淋巴细胞凋亡的发生规律。结果：①10～30mW/cm~2 HPM辐照后,C57小鼠外周血WBC出现降低,而淋巴细胞凋亡率明显升高,与WBC的变化呈较好的对应关系,且在0～15mW/cm~2内呈一定的剂量效应关系。②照后7d,C57小鼠免疫组织淋巴细胞凋亡率均出现明显升高,而在0～15mW/cm~2范围内与辐照剂量呈一定的量效关系。③不同剂量辐照后3d,129小鼠免疫组织淋巴细胞凋亡率均出现明显升高,而以脾组织淋巴细胞凋亡率与剂量呈一定的量效关系。淋巴结经15mW/cm~2辐照后3、7和14 d,其凋亡率的升高与照后时间显示一定的时效关系。结论：虽然对非热效应的生物学影响及其机制仍存在争议,然而本研究结果明确指出,微波对机体免疫机能影响的非热效应还是比较明显的,有关机制尚需进一步阐明。
Objective: To observe the effect of different intensities of high power microwave (HPM) on the immune tissues and peripheral blood lymphocytes of mice and its mechanism. Methods: The apoptosis of thymus, spleen, lymph node and peripheral blood lymphocytes in mice was studied by TUNEL and FCM (Annexin-V / PI double labeling). Results: ① After WBC was treated with 10-30 mW / cm ~ 2 HPM, the WBC in peripheral blood of C57 mice decreased and the rate of lymphocyte apoptosis was significantly increased, which was in good agreement with the change of WBC. At 0-15 mW / cm ~ 2 showed a dose-response relationship. ②After 7 days of irradiation, the apoptosis rate of lymphocytes in C57 mice increased obviously, while there was a certain dose-effect relationship between irradiation dose and 0 ~ 15mW / cm ~ 2. (3) The apoptotic rate of lymphocytes in 129 mice was obviously increased on the 3rd day after irradiation with different dose, but the dose-effect ratio of lymphocyte apoptosis rate in the spleen was dose-dependent. Lymph nodes after 15mW / cm 2 3,7 and 14 d after irradiation, the apoptotic rate increased with the time after irradiation showed a certain aging relationship. CONCLUSION: Although the biological effects of non-thermal effects and their mechanisms remain controversial, the results of this study clearly point out that the non-thermal effects of microwaves on the immune function of the body are still quite obvious, and the relevant mechanisms need to be further elucidated.