采用反相高效液相色谱法测定人血清中异丙酚(Propofol)。血清样品经过C_(18)固相萃取小柱提取,甲醇洗脱,洗脱液在氮气下吹干,残渣用200μl甲醇溶解进样。流动相为乙腈:水=2:1,用高氯酸液调pH=4,检测波长为276nm。异丙酚线性范围为0.5～20.0μg·ml~(-1),r=0.999 0,平均回收率为95.65％,日内和日间RSD范围为2.1～4.1％,最低检测浓度为0·2μg·ml~(-1) Determination of Propofol in Human Serum by Reversed-Phase High Performance Liquid Chromatography. Serum samples were extracted by C_ (18) solid-phase extraction cartridge and eluted with methanol. The eluate was air-dried under nitrogen and the residue was dissolved in 200 μl of methanol. The mobile phase was acetonitrile: water = 2: 1, the pH was adjusted to 4 with perchloric acid solution and the detection wavelength was 276 nm. The linear range of propofol was 0.5 ~ 20.0μg · ml ~ (-1), r = 0.999 0, the average recovery was 95.65%, the RSD ranged from 2.1% to 4.1%, and the lowest detection limit was 0.2μg · ml ~ (-1)