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目的:探讨地塞米松对哮喘小鼠调节激活正常T细胞表达和分泌细胞因子(RANTES)蛋白和m-RNA表达的影响。方法:将30只二级雄性BALB/C小鼠分为正常小鼠组(A组)、哮喘小鼠组(B组)和地塞米松处理组(C组)。以卵清白蛋白(OVA)致敏激发法制备小鼠哮喘模型。采用免疫组化法和原位杂交法测定RANTES蛋白和mRNA在支气管的表达。结果:免疫组化和原位杂交均显示B组支气管RANIES蛋白和mR-NA的表达均显著高于A组(P<0.01),主要表达细胞是上皮细胞,C组支气管RAMES蛋白和mRNA的表达显著低于B组(P<0.01)。结论:RANTES参与哮喘发病机制,上皮细胞是主要的表达细胞;地塞米松对哮喘的拮抗治疗作用部分通过抑制RANTES等趋化因子起作用。
Objective: To investigate the effect of dexamethasone on the expression of activated normal T cells and the expression of RANTES and m-RNA in asthmatic mice. Methods: Thirty male BALB / C mice were divided into normal group (group A), asthma group (group B) and dexamethasone group (group C). Mouse asthma model was induced by ovalbumin (OVA) sensitization. Immunohistochemistry and in situ hybridization were used to determine the expression of RANTES protein and mRNA in bronchus. Results: Immunohistochemistry and in situ hybridization showed that the expression of RANIES protein and mR-NA in bronchus of group B were significantly higher than those in group A (P <0.01). The expression of RAMES protein and mRNA in bronchus of epithelial cells and bronchus in group C Significantly lower than the B group (P <0.01). CONCLUSIONS: RANTES is involved in the pathogenesis of asthma and epithelial cells are the major expressed cells. The antagonistic therapeutic effect of dexamethasone on asthma partly through the inhibition of chemokines such as RANTES.