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目的建立测定大鼠血浆中新人参二醇(NPD)浓度的UPLC-MS/MS检测方法,研究NPD的大鼠血浆蛋白结合率。方法在体外采用平衡透析法模拟NPD体内与血浆蛋白结合的过程,通过UPLC-MS/MS分析测定不同浓度NPD在透析袋内血浆中和透析外缓冲液中的浓度,计算NPD的大鼠血浆蛋白结合率。结果 NPD在0.05~8μg·mL~(-1)内呈现良好的线性关系,质控样品的日内和日间精密度(RSD)均小于15%,方法回收率均高于79.37%;NPD在低、中、高3个浓度水平下的大鼠血浆蛋白结合率分别为(86.55±4.50)%、(76.50±2.61)%和(78.25±1.32)%,各浓度间的结合率数据无显著性差异(P>0.05)。结论 NPD在大鼠体内主要以与血浆蛋白结合的形式存在。
OBJECTIVE To establish a method for the determination of NPD in rat plasma by UPLC-MS / MS and to study the plasma protein binding of NPD in rats. Methods The process of NPD binding to plasma proteins was simulated by equilibrium dialysis in vitro. The concentration of NPD in the dialysis bag plasma and the dialysis buffer was determined by UPLC-MS / MS. The NPD rat plasma protein Binding rate. Results NPD showed a good linear relationship in 0.05 ~ 8μg · mL ~ (-1), the intra-and inter-day precision of RSD was less than 15%, and the recovery rate was higher than 79.37%. NPD was lower (86.55 ± 4.50)%, (76.50 ± 2.61)%, and (78.25 ± 1.32)%, respectively. There was no significant difference in the binding rate between the three concentrations (P> 0.05). Conclusion NPD mainly exists in the form of binding to plasma protein in rats.