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目的研究人肝癌细胞SMMC-7721对米托蒽醌聚乳酸纳米粒(DHAQ-PLA-NP)和米托蒽醌(DHAQ)的敏感性和体外摄取特性。方法采用MTT比色法测定不同剂量的DHAQ-PLA-NP和DHAQ对SMMC-7721的细胞毒作用。SMMC-7721细胞对DHAQ、DHAQ-PLA-NP和2%Dextran 70包裹的DHAQ-PLA-NP的摄取与其浓度和孵育时间、温度有关,用HPLC法测定药物的摄取量。结果 DHAQ-PLA-NP和DHAQ对SMMC-7721均有强的杀伤作用,其杀伤效应呈时间和剂量依赖性;纳米粒组的摄取量高于原药,Dextran 70包裹纳米粒的摄取量也高于普通纳米粒(P<0.05)。结论 DHAQ-PLA-NP可增加SMMC-7721肝癌细胞对药物的摄取,同时,Dextran 70包裹纳米粒后摄取作用更显著。
Objective To investigate the sensitivity and in vitro uptake of mitoxantrone-polylactic acid nanoparticles (DHAQ-PLA-NP) and mitoxantrone (DHAQ) by human hepatoma SMMC-7721 cells. Methods The cytotoxic effect of DHAQ-PLA-NP and DHAQ at different dosages on SMMC-7721 cells was determined by MTT assay. The uptake of DHAQ, DHAQ-PLA-NP and DHAQ-PLA-NP coated with 2% Dextran 70 in SMMC-7721 cells was related to the concentration, incubation time and temperature, and the drug intake was determined by HPLC. Results Both DHAQ-PLA-NP and DHAQ had a strong killing effect on SMMC-7721, and the killing effect was time-and dose-dependent. The uptake of nanoparticles in the nanoparticle group was higher than that of the original drug, and the uptake of Dextran 70-coated nanoparticles was also high In normal nanoparticles (P <0.05). Conclusion DHAQ-PLA-NP can increase the drug uptake in SMMC-7721 hepatocarcinoma cells. In addition, the uptake by Dextran 70 nanoparticles is more pronounced.