应用高效液相色谱法对植物甾醇乙酰化过程的产物进行了测定,实验采用Hypersil ODS反相柱(4.6×150mm,5μm),流动相为甲醇,紫外检测波长为210nm,恒溶剂洗脱的操作条件。实验结果表明:植物甾醇及其乙酸酯的响应时间小于25min,且各种甾醇及其酯的分离效果较好,采用该方法能够快速准确地对植物甾醇酯化反应进行的程度和酯化产物进行定量测定。 The product of acetylation of phytosterols was determined by high performance liquid chromatography (HPLC). The reaction was performed on a Hypersil ODS reverse phase column (4.6 × 150 mm, 5 μm) with a mobile phase of methanol and UV detection wavelength of 210 nm. condition. The experimental results show that the response time of phytosterol and its acetate is less than 25min, and the separation effect of various sterols and esters is good. By using this method, the degree of phytosterol esterification and esterification products Quantitative determination.