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目的探讨建立CM-Dil荧光标记的肝纤维化大鼠骨髓源性内皮祖细胞的方法。方法 Wistar大鼠16只,四氯化碳皮下注射建立肝纤维化大鼠模型,分离培养肝纤维化大鼠骨髓源性内皮祖细胞,分为实验组和对照组,实验组采用CM-Dil荧光进行标记内皮组细胞,对照组未进行标记。采用激光共聚焦显微镜、流式细胞分析仪检测CM-Dil荧光标记率,观察2组细胞生长状态和细胞动力学。结果实验组CM-Dil标记的细胞呈红色荧光,标记阳性率为96.95%;实验组细胞生长状态良好,与对照组比较,生长曲线无明显改变。结论 CM-Dil可在体外成功标记肝纤维化大鼠骨髓源性内皮祖细胞。
Objective To explore the method of establishing CM-Dil fluorescently labeled bone marrow-derived endothelial progenitor cells of rat with hepatic fibrosis. Methods Sixteen Wistar rats were injected subcutaneously with carbon tetrachloride to establish a rat model of hepatic fibrosis. The bone marrow-derived endothelial progenitor cells were isolated and cultured into experimental group and control group. CM-Dil fluorescence Endothelial cells were labeled and control groups were not labeled. The laser confocal microscopy and flow cytometry were used to detect the CM-Dil fluorescent labeling rate. The cell growth status and cell kinetics were observed. Results The CM-Dil-labeled cells in the experimental group showed red fluorescence with a positive rate of 96.95%. The growth of cells in the experimental group was good. Compared with the control group, the growth curve did not change significantly. Conclusion CM-Dil can successfully label bone marrow-derived endothelial progenitor cells of rat with hepatic fibrosis in vitro.