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目的:对PFGE和MLST方法在阪崎肠杆菌分型研究中的分辨力和潜在价值进行了比较研究和论述。方法:采用PFGE和MLST方法对本实验室分离的19株阪崎肠杆菌和一株标准菌株ATCC51329进行分型研究。结果:PFGE方法可将20株阪崎肠杆菌分为6大类群,共16个PFGE型,分辨力为0.9474。而MLST方法可将20株菌株分为12个ST型,分辨力为0.9263。结论:PFGE法较MLST法具有较高的分辨力,可用于阪崎肠杆菌的溯源研究,而MLST分型方法能通过全球比对数据库得到更多的关于进化和亲缘关系的分析资料,在致病研究、流行病学调查、进化研究方面优于PFGE法。
Objective: To compare and discuss the discrimination and potential value of PFGE and MLST in the typing of Enterobacter sakazakii. Methods: 19 strains of Enterobacter sakazakii isolated from our laboratory and one standard strain ATCC51329 were genotyped by PFGE and MLST. Results: PFGE method could classify 20 strains of Enterobacter sakazakii into six groups, with a total of 16 PFGE types with a resolution of 0.9474. The MLST method can be divided into 12 strains of ST strains with a resolution of 0.9263. Conclusion: The PFGE method has higher resolution than MLST method, which can be used for the traceability of Enterobacter sakazakii. However, the MLST typing method can get more information about evolution and kinship through the global database. Disease research, epidemiological investigation, evolutionary research is superior to the PFGE method.