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人体组织液中的葡萄糖浓度与血糖浓度密切相关,微创血糖检测通过测量组织液中的葡萄糖浓度来预测血糖浓度,而组织液中成分复杂,利用对葡萄糖有特异性吸附作用的D-半乳糖/D-葡萄糖结合蛋白(GGBP)胺耦合绑定修饰表面等离子共振(SPR)传感器可以实现葡萄糖选择性测量.但是由于测量过程复杂,需要对影响测量精度的因素进行评价,确定最佳测量条件,以微型SPR传感器为核心搭建了一套葡萄糖浓度测量系统,系统地分析研究了GGBP胺耦合绑定SPR葡萄糖浓度测量过程中的各种影响因素,在不同实验条件下进行了测量实验,获得了蛋白质纯化、蛋白质活性、基线、流速、传质效应、温度和气泡等对测量结果的影响机制,提出了相应的解决方案,优化了测量流程,为标准化和小型化仪器开发提供重要依据.
Glucose concentration in human tissue fluid is closely related to blood glucose concentration, which is estimated by measuring the glucose concentration in tissue fluid, while the composition of tissue fluid is complex. Using D-galactose / D- Glucose binding protein (GGBP) amine coupled binding modified surface plasmon resonance (SPR) sensor can achieve glucose selectivity measurement.But due to the complexity of the measurement process, the factors that affect the measurement accuracy need to be evaluated to determine the optimal measurement conditions to micro SPR Sensor set as the core of a glucose concentration measurement system, a systematic analysis of the GGBP amine binding binding SPR glucose concentration in the measurement of various influencing factors in different experimental conditions were measured experiments obtained protein purification, protein The mechanism of influence on the measurement results such as the activity, the baseline, the flow rate, the mass transfer effect, the temperature and the bubble, and the corresponding solutions are put forward. The measurement procedure is optimized, which provides an important basis for the development of standardized and miniaturized instruments.