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目的:考察土壤铜污染对青蒿生长及青蒿素含量的影响。方法:在土壤中加入不同浓度的Cu(NO3)2.3H2O,设置土壤中铜浓度为100,300,600 mg.kg-1后播种青蒿种子,观察青蒿在不同浓度Cu土壤中的生长情况,并在80,140 d记录青蒿生长的各项生理指标及在140 d收获时青蒿素含量的变化。结果和结论:在青蒿生长的幼苗期,铜处理组青蒿比空白组长势都较差;青蒿播种80 d后,低浓度铜污染(100 mg.kg-1)对青蒿的影响主要表现为刺激其生长,较高浓度(300 mg.kg-1)和高浓度组(600 mg.kg-1)则主要表现为抑制作用;140 d收获时,铜处理组青蒿长势较好且生物量较对照组高,初步推断可能与青蒿本身的生长发育规律有关;铜处理组明显抑制了青蒿根部的生长;铜主要蓄积在青蒿的根部,根是铜毒害的主要器官;铜低浓度组青蒿素含量比对照组稍高,随着铜浓度的增大,青蒿素的含量逐渐降低,综合考虑青蒿生长期导致的变化,分析原因是过量的铜本身抑制青蒿素的合成与积累。
Objective: To investigate the effects of soil copper pollution on the growth and artemisinin content of Artemisia annua. Methods: Different concentrations of Cu (NO3) 2.3H2O were added to the soil. The seeds of Artemisia annua L. were sowed after the concentration of Cu was 100, 300 and 600 mg.kg-1 in the soil. The growth of Artemisia annua was observed in different concentrations of Cu soils. d record each physiological growth of Artemisia annua L. and the change of artemisinin content at 140 d harvest. RESULTS AND CONCLUSION: Artemisia annua L. in the copper treatment group had a poorer growth rate than the blank group at the seedling stage of Artemisia annua L. The effect of low concentration copper contamination (100 mg.kg-1) on Artemisia annua was mainly The results showed that the growth of plants was mainly inhibited by the high concentration (300 mg · kg-1) and 600 mg · kg-1 (600 mg · kg-1) The biomass was higher than that of the control group. The preliminary inference may be related to the growth and development of Artemisia annua L. The copper treatment significantly inhibited the growth of A. annua. The main copper accumulation was in the root of A. annua and the root was the main organ of copper toxicity. The content of artemisinin in the low concentration group was slightly higher than that in the control group. The content of artemisinin gradually decreased with the increase of copper concentration, and the reason was that the excessive copper itself suppressed the inhibition of artemisinin The synthesis and accumulation.