论文部分内容阅读
为研究卵巢癌盆腔引流淋巴结的淋巴细胞,经CD3单克隆抗体激活后的杀伤细胞CD3AK在体外的抗瘤作用.作者采用人卵巢癌病人盆腔引流淋巴结的淋巴细胞,在体外与CD3单抗和少量rIL-2诱导并激活后,在我所自建的两株人卵巢癌HO-891O、HO-89lOPM细胞系上进行了研究.两株细胞各随机分成6组:1)阴性对照组(A组):只加新鲜的全培养液 ; 2)低浓度CD3AK组(B组):5×10~5/ml CD3AK培养液;3)中浓度CD3AK组(C组):1×10~6/mlCD3AK培养液;4)高浓度CD3AK组(D组):2.5×10~6/mlCD3AK培养液;5)顺铂阳性对照组(E组):含10μg/ml顺铂的培养液;6)顺铂+CD3AK联合用药组(F组):含10μg/ml顺铂+1×10~6/mlCD3AK培养液,分别用LDH活性测定、自然杀伤率及台盼兰活细胞计数法观察结果.结果表明:CD3AK细胞对卵巢癌细胞有明显的杀伤作用,两株细胞均以中浓度CD3AK细胞
In order to study the anti-tumor effect of CD3AK, a killer cell activated by CD3 monoclonal antibody in lymph node of pelvic draining lymph node of ovarian cancer in vitro, we used lymphocyte of pelvic draining lymph node in human ovarian cancer patients, Two human ovarian cancer HO-891O and HO-89lOPM cell lines were studied after induction and activation of rIL-2.The two cells were randomly divided into 6 groups: 1) negative control group (group A ): Only fresh whole broth; 2) low concentration of CD3AK group (group B): 5 × 10-5 / ml CD3AK medium; 3) medium concentration of CD3AK group (group C): 1 × 10-6 / mlCD3AK Culture medium; 4) high concentration CD3AK group (group D): 2.5 × 10 ~ 6 / mlCD3AK medium; 5) cisplatin positive control group (group E) + CD3AK combination group (F group): 10μg / ml cisplatin + 1 × 10 ~ 6 / mlCD3AK culture medium, respectively, the determination of LDH activity, natural killer rate and trypan blue survival cell count results showed that: CD3AK cells have a significant killing effect on ovarian cancer cells, both cells were medium concentrations of CD3AK cells