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目的 :动态研究黄芪注射液对人巨细胞病毒 (HCMVAD1 6 9)感染的人脐血粒 -单系祖细胞 (CFU-GM)体外增殖的影响。方法 :隔日计HCMVAD1 6 9体外持续感染后 1～ 19天内黄芪注射液对CFU -GM细胞簇、集落、大集落及细胞总数变化规律。结果 :培养后各组CFU -GM增殖均有不同程度的增加 ,7天达峰后减少。病毒组CFU -GM增殖均较空白组有明显降低 (P <0 0 5 ) ;黄芪组、更昔洛韦组和空白组细胞增殖明显高于病毒组 (P <0 0 1) ;更昔洛韦组和黄芪组CFU -GM增殖显著高于病毒组 (P <0 0 5 )。结论 :黄芪注射液可以促进体外培养HCMV感染CFU -GM的增殖。
Objective : To study the effect of Huangqi injection on the proliferation of human umbilical blood mononuclear cells (CFU-GM) infected with human cytomegalovirus (HCMVAD169) in vitro. METHODS: The changes of CFU-GM cell clusters, colonies, large colonies, and total number of cells were observed within 1 to 19 days after continuous infection with HCMVAD1 6 9 in vitro. Results: The proliferation of CFU-GM in all groups increased in different degrees after culture, and decreased after reaching the peak in 7 days. The proliferation of CFU-GM in the virus group was significantly lower than that in the blank group (P < 0.05); the cell proliferation in the Astragalus, ganciclovir, and blank groups was significantly higher than that in the virus group (P <0 01); The proliferation of CFU-GM in Wei group and Huangqi group was significantly higher than that in virus group (P<0 05). Conclusion: Huangqi injection can promote the proliferation of HCMV-infected CFU-GM in vitro.