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目的:利用髓性白血病细胞HL60细胞,研究低剂量电离辐射(1Gy)对MAPK(丝裂原激活的蛋白激酶)和JNK(c-JUN末端激酶)活性的影响,确定MAPK介导与细胞辐射敏感性以及bcl-2表达与细胞存活的关系。方法:在辐照前和辐照中用MEK1/2(MAPK激酶)的抑制剂PD98059处理转化的HL60/pCEP4(对照质
OBJECTIVE: To study the effect of low dose ionizing radiation (1Gy) on the activity of MAPK (mitogen-activated protein kinase) and JNK (c-JUN terminal kinase) in HL60 cells of myeloid leukemia and to determine the effect of MAPK-mediated and cell-radiation sensitive Sex and the relationship between bcl-2 expression and cell survival. Methods: Transformed HL60 / pCEP4 (control material) was treated with inhibitor PD98059 of MEK1 / 2 (MAPK kinase) before and during irradiation