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首次使用甲基化敏感扩增多态性(MSAP)技术分析两种小麦材料近等基因系TcLr19及其感病亲本Thatcher的甲基化水平,同时比较了苗期接种叶锈菌生理品种THTT前后基因组DNA胞嘧啶甲基化模式。60对选扩引物对接种前后的小麦DNA进行全基因组筛选,共得到3 554个片段。其中998个片段是两种甲基化模式中的一种,小麦近等基因系TcLr19及其感病亲本Thatcher的甲基化水平约为28.1%。在所有的引物中,并没有直接分离得到接菌前后的甲基化模式的差异。结果初步表明,叶锈菌可能并没有诱导植物基因组DNA胞嘧啶位点的甲基化模式变化。
For the first time methylation-sensitive amplification polymorphism (MSAP) was used to analyze the methylation level of TcLr19 and its susceptible parent Thatcher in two wheat materials. Genomic DNA cytosine methylation patterns. 60 pairs of primers were screened for whole genome of wheat DNA before and after inoculation, a total of 3 554 fragments were obtained. Among them, 998 fragments were one of two methylation patterns. The methylation level of wheat near-isogenic line TcLr19 and its susceptible parent Thatcher was about 28.1%. Among all the primers, the differences in methylation patterns before and after inoculation were not isolated directly. Preliminary results showed that leaf rust may not induce changes in methylation patterns of cytosine sites in plant genomic DNA.