作者研究前向运动精子在含4.7或25mM钾的培养介质中培养24小时后的变化,介质中分别加入或不加仓鼠卵丘。在有卵丘复合物中24小时,精子的前向运动百分比明显增高,与钾浓度无关。在含25mM钾及卵丘中精子死亡率明显降低。不论时间长短及有无卵丘,在25mM钾中比4.7mM钾培养的精子顶体反应百分率较高,在2及5小时培养后穿卵率也较高。培养介质中有卵丘者,穿透卵的百分 The authors studied the changes in motile spermatozoa cultured in culture medium containing 4.7 or 25 mM potassium for 24 hours, with or without Hamster cumulus. In the cumulus complex 24 hours, the percentage of sperm forward movement was significantly increased, and has nothing to do with the concentration of potassium. Sperm mortality was significantly lower in 25 mM potassium and cumulus. The percentage of sperm acrosome reaction was higher in 25 mM potassium than 4.7 mM potassium irrespective of length of time and presence of cumulus, and the rate of ovulation was also higher after 2 and 5 hours incubation. Culture medium in the cumulus who penetrate the percentage of eggs