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目的 了解 p16 (MTS1)和 p15 (MTS2 )基因异常与小儿急性淋巴细胞白血病 (ALL)发生的关系。方法 用聚合酶链反应 (PCR)和Southernblot杂交法检测了 2 1例小儿ALL患者骨髓细胞 p16和 p15基因的缺失情况 ;用逆转录 聚合酶链反应 (RT PCR)技术检测了 19例患儿 p15mRNA表达。结果 经PCR检测 ,p16基因外显子 1,2均缺失者 8例 (38 1% ) ,p15基因外显子1,2均缺失者为 11例 (5 2 3% )。Southernblot检测的结果与PCR的结果相一致。RT PCR检测发现 2例 p15基因不缺失患儿的样品无p15mRNA表达。 结论 p16和p15基因在ALL患儿中存在高频率缺失和转录异常 ,提示这两种基因在ALL患儿的淋巴细胞恶性转化中起重要作用
Objective To investigate the relationship between the abnormalities of p16 (MTS1) and p15 (MTS2) genes and the occurrence of childhood acute lymphoblastic leukemia (ALL). Methods The deletion of p16 and p15 genes in bone marrow cells from 21 pediatric ALL patients were detected by polymerase chain reaction (PCR) and Southern blot hybridization. The expression of p15 mRNA in 19 children was detected by reverse transcriptase-polymerase chain reaction (RT PCR) expression. Results There were 8 cases (38 1%) in which p16 gene exon 1 and 2 were deleted and 11 cases (5 23%) in exon 1 and 2 of p15 gene were detected by PCR. The result of Southern blotting is consistent with the result of PCR. RT-PCR detection found no p15 gene expression in 2 samples of children without deletion of p15 gene. Conclusion There is a high frequency of deletion and transcriptional abnormalities of p16 and p15 genes in children with ALL, suggesting that these two genes play an important role in the malignant transformation of lymphocytes in children with ALL