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目的用代谢组学研究儿童真性性早熟(CPP)的代谢标记物,以探讨儿童性早熟的发病机制。方法用高效液相色谱-四级杆飞行时间质谱联用系统(HPLC-QTOF-MS)对性早熟组和对照组儿童的血液样本进行代谢图谱分析,并结合主成分分析法(PCA)和偏最小二乘辨别分析法(PLS-DA),根据PLS分析的载荷图和VIP值,筛选24个代谢标记物,并对其参与的代谢途径进行分析。结果氨基酸、5-羟色胺、儿茶酚胺及脂代谢等多条代谢途径异常与儿童性早熟发生相关。此外,一些环境内分泌干扰物也可能导致性早熟。结论性早熟儿童和正常儿童血液代谢特征存在明显差异。
Objective To study the metabolic markers of childhood precocious puberty (CPP) with metabolomics to explore the pathogenesis of precocious puberty in children. Methods The blood samples of precocious and control children were analyzed by HPLC-QTOF-MS and their metabolites were analyzed by principal component analysis (PCA) and partial PLS-DA, 24 metabolic markers were screened based on PLS-analyzed load maps and VIP values, and their metabolic pathways were analyzed. Results Amino acids, serotonin, catecholamines and lipid metabolism and other metabolic abnormalities associated with precocious puberty in children. In addition, some environmental endocrine disruptors may also cause precocious puberty. Conclusions There are significant differences in blood metabolic characteristics among precocious and normal children.