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引言研究反刍动物需要的饲草营养价值时,使植物代谢作用和细胞呼吸作用减低到最低程度,才能确保分析结果精确地反映被食植物的营养成分。研究草本植物不同部分的消化率也需如此(Burns,1981)。研究者经常从远离实验室的地点采样,如果刈割牧草样品后能就地冷冻,细胞代谢作用在数小时内即可减低至最低程度。用液N快速冷冻植物新鲜组织,减低酶活性的方法是适用的(Noller等1966;Bums,
Introduction Research on the nutritional value of forages required by ruminants minimizes plant metabolism and cellular respiration in order to ensure that the analytical results accurately reflect the nutritional content of the plants being eaten. This is also the case for studying the digestibility of different parts of the herb (Burns, 1981). Researchers often sample from far away from the laboratory, and if they are frozen in situ after mowing pasture samples, cellular metabolism can be reduced to a minimum within hours. Methods for rapidly freezing plant fresh tissue with liquid N to reduce enzyme activity are suitable (Noller et al 1966; Bums,