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目的:探讨维生素B6对大鼠实验性肝癌形成过程中脂质过氧化的影响。方法:雄性SD大鼠随机分为三组。正常组大鼠喂普通饲料;DAB组大鼠饲料中含0.06%3′-甲基-4-双甲氨基偶氮苯(3′-MeDAB),10周后改喂普遍饲料;B6组大鼠饲料中除含有0.06%3′-MeDAB外,另加Vit.B6800mg/kg,10周后停喂3′-MeDAB,改喂加入800mg/kgVit.B6的普通饲料。动态观察大鼠全血GSH-Px的活性、血清MDA含量的改变。结果:(1)给予3′-MeDAB后,大鼠血中GSH-Px活性降低,MDA含量增加,与正常组相比有显著差异(P<0.01)。添加维生素B6后,在第45天,GSH-Px活性比DAB组明显提高(P<0.01),MDA含量明显降低(P<0.05)。(2)第45天时,DAB组的肝脏脏器系数已显著高于正常组(P>0.05)。结论:在实验性肝癌的早期,维生素B6有提高大鼠体内抗氧化酶活性、减少脂质过氧化物的作用,从而在一定程度上减轻致癌剂对大鼠肝脏的损害。
Objective: To investigate the effect of vitamin B6 on lipid peroxidation during the formation of experimental liver cancer in rats. Methods: Male SD rats were randomly divided into three groups. The rats in normal group were fed with normal diet. The feed of DAB group contained 0.06% 3’-methyl-4-dimethylaminoazobenzene (3’-MeDAB), and fed to general feed after 10 weeks. Rats in group B6 In addition to feed containing 0.06% 3’-MeDAB, plus Vit.B6800mg / kg, 10h stop feeding 3’-MeDAB, change to feed 800mg / kgVit.B6 common feed. Dynamic observation of rat whole blood GSH-Px activity, serum MDA content changes. Results: (1) After given 3’-MeDAB, the activity of GSH-Px in the blood of rats decreased and MDA content increased, which was significantly different from that of the normal group (P <0.01). After adding vitamin B6, the activity of GSH-Px was significantly increased (P <0.01) and the content of MDA was significantly decreased on the 45th day (P <0.05). (2) On the 45th day, the liver organ coefficient of DAB group was significantly higher than that of the normal group (P> 0.05). Conclusion: In the early stage of experimental liver cancer, vitamin B6 can improve antioxidant enzyme activity in rats and reduce the role of lipid peroxides, thereby reducing the damage to rat liver to a certain extent.