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目的探讨清开灵注射液对全氟异丁烯(PFIB)吸入性急性肺水肿的治疗作用。方法小鼠以全身暴露动态吸入PFIB方式染毒,剂量为130 mg/m3染毒5 m in。在小鼠PFIB染毒后1 h腹腔注射清开灵注射液,剂量分别为1.75、3.50、7.00、14.00 m l/kg。以染毒小鼠存活率、肺系数、支气管肺泡灌洗液(BALF)中蛋白含量及弹性蛋白酶活力、组织病理学检查等为观测指标,观察清开灵注射液抗PFIB吸入性急性肺水肿作用的量效关系。结果清开灵注射液可显著降低PFIB染毒小鼠的湿(干)肺体比、肺含水量及肺湿/干比,明显降低BALF中的蛋白含量,显著提高PFIB染毒小鼠的存活率,改善染毒小鼠的肺组织病变,且量效关系良好。结论清开灵注射液对PFIB所致肺水肿与炎性渗出具有肯定的抑制作用,并能有效对抗PFIB所致动物死亡。
Objective To investigate the therapeutic effect of Qingkailing injection on acute pulmonary edema induced by perfluoroisobutylene (PFIB) inhalation. Methods Mice were exposed to PFIB inhalation with systemic exposure at a dose of 130 mg/m3 for 5 min. Qingkailing injection was injected intraperitoneally 1 hour after the mice were exposed to PFIB. The doses were 1.75, 3.50, 7.00, and 14.00 ml/kg, respectively. The effect of Qingkailing injection on acute pulmonary edema induced by PFIB inhalation was observed with the survival rate, lung coefficient, protein content in bronchoalveolar lavage fluid (BALF), elastase activity and histopathological examination. The dose-effect relationship. Results Qingkailing injection can significantly reduce the wet (dry) lung ratio, lung water content and lung wet/dry ratio in PFIB-treated mice, significantly reduce the protein content in BALF, and significantly increase the survival of PFIB-exposed mice. Rate, to improve the lung tissue lesions of mice infected with, and the dose-effect relationship is good. Conclusion Qingkailing injection has a positive inhibitory effect on pulmonary edema and inflammatory exudation caused by PFIB, and can effectively combat animal death caused by PFIB.