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应用３对人乳头瘤病毒（ＨＰＶ）引物对１０７例各种宫颈标本进行了聚合酶链反应（ＰＣＲ）扩增。结果显示，共同引物（ＧＰ）扩增，有５８．８％（３０／５１）宫颈鳞癌，１００％（１４／１４）尖锐湿疣、１３．６％（３／２２）宫颈炎和１０％（２／２０）正常宫颈出现ＨＰＶ阳性。型特异性引物ＳＰ１６／ＳＰ１８扩增，有３７．２％（１９／５１）宫颈鳞癌２５．７％（５／１４）尖锐湿疣和５％（１／２０）正常宫颈出现ＨＰＶ１６型阳性，５．８％（３／５１）宫颈鳞癌为ＨＰＶ１８型阳性。进一步用ＳＰ１６ｂ引物扩增，没有１例ＨＰＶ１６ｂ亚型被发现。说明宜颈磷癌和尖锐湿疣与ＨＰＶ感染有关，结合应用共同引物和型特异性引物可作为ＨＰＶ检测与分型方法。
Application of three pairs of human papillomavirus (HPV) primers in 107 cases of various cervical specimens by polymerase chain reaction (PCR) amplification. The results showed that there were 58.8% (30/51) cervical squamous cell carcinoma, 100% (14/14) condylomata acuminata, 13.6% (3/22) cervicitis and 10% 2/20) HPV positive in normal cervix. The positive rate of HPV16 was 25.2% (19/51) in cervical squamous cell carcinoma and 5% (1/20) in normal cervix. .8% (3/51) cervical squamous cell carcinoma is HPV18 positive. Further amplification with SP16b primers, none of the HPV16b subtypes were found. Description of cervical cancer and genital warts and HPV infection, combined with the use of common primers and type-specific primers can be used as HPV detection and typing methods.