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遗传基因的分离需要供、受体亲本间较大的遗传差异.该文利用均匀分布于水稻12条染色体的429个SSR标记,分析了日本晴与5个自育优良恢复系之间的多态性标记及分布密度,进而评价它们间的遗传差异性.结果显示,日本晴与西恢18号、缙恢35、R225、R232和R250之间的多态性标记数分别为263,253,253,255和240个;多态率分别为61.31%,58.97%,58.97%,59.44%和55.94%;每个标记间的平均物理距离分别为1.59,1.61,1.62,1.62和1.72 Mb.每个SSR位点可检测到的等位基因数目为2~5个,平均每对SSR引物检测到2.35个等位基因.各亲本之间的遗传距离在0.121 7~0.896 7之间,日本晴与5个恢复系间的平均遗传距离为0.857 6.聚类分析也将日本晴与5个恢复系分为两大亚类,表明它们之间存在极大的遗传差异.这些材料和结果对水稻遗传基因的分离具有重要意义.
Genetic segregation requires large genetic differences between donor and recipient parents.In this study, 429 SSR markers that were evenly distributed on 12 chromosomes of rice were used to analyze the polymorphism between Nipponbare and five self-reclaimed elite restorer lines The results showed that the number of polymorphic markers between Nipponbare and Xihui 18, Jinhui 35, R225, R232 and R250 were 263, 253, 253, 255 and 240, respectively. Polymorphism The average physical distances between each marker were 1.59, 1.61, 1.62, 1.62 and 1.72 Mb, respectively. The detectable alleles at each SSR locus were 61.31%, 58.97%, 58.97%, 59.44% and 55.94% The number of genes was 2 ~ 5, with an average of 2.35 alleles per pair of SSR primers.The genetic distance between parents was between 0.121 7 and 0.896 7, and the average genetic distance between Nipponbare and five restorer lines was 0.857 6. Cluster analysis also separates Nipponbare and five restorer lines into two major subgroups, indicating that there is a great genetic difference between them. These materials and results are of great significance for the isolation of rice genetic elements.