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目的：研究一氧化氮（ＮＯ）对体外培养脊髓及背根神经节细胞的作用．方法：应用ＭＴＴ微量比色法观察ＮＯ研究工具药Ｌ┐精氨酸（Ｌ┐Ａｒｇ）和Ｌ┐Ｎ位硝基精氨酸（Ｎ┐Ａｒｇ）对脊髓及背根神经节细胞体外存活和突起生长的影响．结果：一氧化氮合酶（ＮＯＳ）底物Ｌ┐Ａｒｇ（５００、１０００μｍｏｌ／Ｌ）明显抑制体外培养脊髓及背根神经节细胞的存活和突起生长，降低其活性，而ＮＯＳ抑制剂Ｎ┐Ａｒｇ（２００μｍｏｌ／Ｌ）则表现出相反的作用，并可逆转Ｌ┐Ａｒｇ的作用．结论：ＮＯ抑制脊髓及背根神经节细胞的体外存活和突起生长，ＮＯＳ抑制剂Ｎ┐Ａｒｇ则有一定的细胞保护作用．
Objective: To study the effect of nitric oxide (NO) on cultured spinal cord and dorsal root ganglion cells in vitro. Methods: MTT microtitre assay was used to observe the survival and neurite outgrowth of spinal cord and dorsal root ganglion (DRG) neurons in vitro by L-arginine (L┐Arg) and L┐N-nitro arginine (N┐Arg) The impact of growth. Results: L┐Arg (500,1000 μmol / L), a nitric oxide synthase (NOS) substrate, significantly inhibited the survival and proliferation of cultured spinal cord and dorsal root ganglion cells in vitro and decreased their activities. However, N ┐ Arg (200μmol / L) showed the opposite effect and reversed the effect of L┐Arg. CONCLUSION: NO inhibits the survival and neurite outgrowth of spinal cord and dorsal root ganglion cells in vitro, and N? Arg inhibitor N? Arg has certain cytoprotective effects.