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为优化大豆体细胞胚再生植株的条件,提高大豆遗传转化的效率,本研究选用大豆品种合丰25为试验材料,针对影响体细胞胚诱导的3个关键因素pH值、2,4-D浓度及光照条件进行体细胞胚发生频率的研究。结果表明:pH值、2,4-D浓度、光照条件这3种因素的不同处理对体细胞胚的诱导影响显著,其幼胚的发生频率最高为70.70%、最低为3.70%。当pH值为7.0、2,4-D浓度为20mg/L,黑暗培养最有利于合丰25体细胞胚的诱导,其胚的发生频率为70.70%,明显高于其他处理。
In order to optimize the conditions of soybean somatic embryos regenerated plants and improve the efficiency of soybean genetic transformation, this study selected soybean variety Hefeng 25 as test material, and studied the effects of three key factors of somatic embryo on pH value, 2,4-D concentration And light conditions for the study of somatic embryogenesis frequency. The results showed that the effects of pH, 2,4-D concentration and light on the induction of somatic embryo were significant. The frequency of embryogenesis was 70.70% and the lowest was 3.70%. When the pH was 7.0 and the concentration of 2,4-D was 20mg / L, the dark culture was the most conducive to the induction of Hefeng25 somatic embryos. The frequency of embryogenesis was 70.70%, which was significantly higher than other treatments.