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目的 :研究高密度脂蛋白 (HDL)与动脉粥样硬化发生的关系。方法 :用 10 %胆固醇脂肪乳剂复制大鼠和家兔的高脂血症模型 ,用HE染色、组织化学、免疫组织化学和ELISA等技术 ,检测比较两种动物动脉粥样硬化形成的情况和血清中的脂蛋白及总胆固醇水平 ,和TGF - β1、 2、 3及其受体的表达状态。结果 :实验组家兔 10 0 %形成动脉粥样斑 ,血清高密度脂蛋白在正常范围 ;实验组大鼠未见动脉粥样斑形成 ,但其血中HDL随实验时间 (鼠龄 )的延长而升高 ;TGF - β及其受体在两种动物间无明显差异。结论 :HDL有保护血管壁内皮细胞不被高脂血症损伤的作用 ;而TGF - β仅在动脉内膜损失后的炎症性增生反应过程中起作用。
Objective: To study the relationship between high-density lipoprotein (HDL) and atherosclerosis. Methods: Hyperlipidemia rats and rabbits were replicated with 10% cholesterol lipid emulsion. HE staining, histochemistry, immunohistochemistry and ELISA were used to detect the formation of atherosclerosis in both animals and serum Lipoprotein and total cholesterol levels, and TGF - β1, 2, 3 and its receptor expression status. Results: Atherosclerotic plaque and serum high density lipoprotein (LDL) were found in 10% of experimental rabbits in the normal range. The formation of atherosclerotic plaque was not observed in the experimental group, but the blood HDL was prolonged with the experimental time While the level of TGF - β and its receptor did not differ between the two animals. CONCLUSION: HDL protects vascular endothelial cells from hyperlipidemia. TGF - β only plays an important role in the process of inflammatory hyperplasia after intimal loss.