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目的探讨层粘素(laminin,LN)及其受体(laminin receptor,LNR)在人卵巢癌细胞黏附和转移中的作用。方法采用细胞黏附实验检测层粘素及其受体在5株卵巢癌细胞黏附中的作用;Boyden小室测定癌细胞侵袭能力;流式细胞技术检测层粘素及其受体在人卵巢癌细胞上的表达情况。结果抗层粘素及其受体抗体,可明显抑制人卵巢癌细胞的黏附作用,且随着稀释度的不同,其抗黏附能力各异。抗层粘素抗体侵袭力测定显示,A-2780最弱,Skov-3最强,两者比较,差异有显著意义(P<0.01)。在5种卵巢癌细胞中,4株癌细胞有层粘素及其受体表达,1株癌细胞无表达。结论层粘素及其受体可通过诱导肿瘤细胞黏附和迁移,从而促进肿瘤细胞浸润和转移。
Objective To investigate the role of laminin (LN) and laminin receptor (LNR) in the adhesion and metastasis of human ovarian cancer cells. Methods Cell adhesion assay was used to detect the role of laminin and its receptors in the adhesion of 5 ovarian cancer cells. Boyden chamber was used to determine the invasiveness of cancer cells. Flow cytometry was used to detect laminin and its receptors on human ovarian cancer cells The expression of the situation. Results Anti-laminin and its receptor antibodies, can significantly inhibit the adhesion of human ovarian cancer cells, and with the dilution of different anti-adhesion ability. Anti-laminin antibody invasion assay showed that A-2780 was the weakest, Skov-3 was the strongest, the difference between the two was significant (P <0.01). Among the five kinds of ovarian cancer cells, four cancer cells have laminin and its receptor expression, but none of the cancer cells have no expression. Conclusion Laminin and its receptor can promote tumor cell infiltration and metastasis by inducing tumor cell adhesion and migration.