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目的研究柚皮苷对蛛网膜下腔出血(subarachnoid hemorrhage,SAH)引起的脑组织神经炎症的影响。方法 54只SD大鼠随机分为假手术组、模型组和柚皮苷组(80 mg·kg~(-1))。各组大鼠过量麻醉致死,断头取脑后干湿重法测水肿;取冻存于液氮的患侧大脑皮质,称重后用裂解液匀浆处理;离心提蛋白,Western blot定量检测炎症相关因子NF-κB;各组大鼠断头取脑后置于4%中性甲醛固定,常规石蜡包埋和切片,免疫荧光染色半定量检测小胶质细胞标记钙离子结合调节因子(ionized calcium-binding adapter molecule 1,Iba-1)和星形细胞标记胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)。结果与模型组比较,柚皮苷能明显减少SAH后脑组织水肿(P<0.05)、NF-κB蛋白表达(P<0.05)、抑制大脑皮质小胶质细胞Iba-1和星形胶质细胞GFAP的活化(P<0.05)。结论柚皮苷在SAH中有明显的抗炎作用。
Objective To study the effect of naringin on neuroinflammation in brain tissue induced by subarachnoid hemorrhage (SAH). Methods 54 SD rats were randomly divided into sham operation group, model group and naringin group (80 mg · kg -1). Rats in each group were over-anesthetized and lethally edema was induced by decapitation and brainstem wet-dry method. The cerebral cortex was frozen in liquid nitrogen and homogenized with lysate. The proteins were extracted by centrifugation and quantified by Western blot Inflammation-related factor NF-κB; rats in each group were decapitated and placed in 4% neutral formalin, embedded in paraffin and sectioned, immunofluorescence staining semi-quantitative detection of microglial markers of ionizing ionized calcium-binding adapter molecule 1, Iba-1) and astrocyte-labeled glial fibrillary acidic protein (GFAP). Results Compared with the model group, naringin could significantly reduce the brain edema (P <0.05) and the expression of NF-κB protein (P <0.05) after SAH, inhibit the expression of Iba-1 and astrocyte GFAP in cerebral cortex (P <0.05). Conclusion Naringin has obvious anti-inflammatory effect in SAH.