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目的通过饮食控制碘铁摄入量,建立碘铁联合缺乏的大鼠动物模型。方法选择健康SPF/VAF级初断乳SD雄性大鼠22只,按体重随机分为对照组(饲以改良的AOAC饲料,碘含量为280μg/kg,铁含量为50mg/kg),缺碘缺铁组(饲以改良的AOAC缺碘缺铁饲料,碘含量为66μg/kg,铁含量为15mg/kg),每组11只。饲养4周后,测定体重和甲状腺重量,并计算甲状腺系数。测定大鼠尿碘、血清甲状腺激素、血红蛋白、血清铁水平和总铁结合力。结果缺碘缺铁组大鼠皮肤苍白,皮毛粗糙稀疏。与对照组比较,缺碘缺铁组大鼠体重、尿碘、血红蛋白、血清铁、血清游离三碘甲腺原氨酸(fT3)、游离甲状腺素(fT4)水平均较低,甲状腺重量及其脏器系数、总铁结合力和fT3/fT4比值均较高,差异有统计学意义(P<0.05);促甲状腺激素(TSH)水平有升高趋势,但差异无统计学意义。结论该模型同时具有碘缺乏和铁缺乏特征,通过饮食控制大鼠碘铁摄入量成功制备碘铁联合缺乏大鼠动物模型。
OBJECTIVE To establish an animal model of iodine-iron deficiency in rats by controlling iodine intake through diet. Methods Twenty-two male Sprague-Dawley (SD) weaned SPF / VAF weaned SD rats were randomly divided into control group (fed with modified AOAC diet, 280μg / kg iodine content and 50mg / kg iron content) Fe group (fed with a modified AOAC diet containing iodine deficient iron, 66 μg / kg iodine, and 15 mg / kg iron), 11 in each group. After 4 weeks of feeding, body weight and thyroid weight were measured, and thyroid factor was calculated. Urinary iodine, serum thyroid hormone, hemoglobin, serum iron level and total iron binding capacity were determined. Results In iodine deficient iron-deficient rats, the skin of the rats was pale and the fur was rough and sparse. Compared with the control group, body weight, urinary iodine, hemoglobin, serum iron, serum free triiodothyronine (fT3) and free thyroxine (fT4) in the iodine deficiency and iron deficient rats were all lower. The thyroid weight and its Organ coefficient, total iron binding capacity and fT3 / fT4 ratio were higher, the difference was statistically significant (P <0.05); thyroid stimulating hormone (TSH) levels increased, but the difference was not statistically significant. Conclusion The model has the characteristics of both iodine deficiency and iron deficiency. The animal model of iodine deficiency and iron deficiency in rats was successfully established by dietary control of iodine intake.