论文部分内容阅读
目的:以欧洲花楸悬浮培养细胞(SASC)为材料,研究非生物诱导子-重金属对SASC生物量的影响。方法:配制不同浓度的氯化镧、硝酸铜、硝酸铅溶液对SASC进行处理,用电子天平进行SASC生物量测定。结果:低浓度氯化镧刺激细胞生长而高浓度则抑制细胞生长,对细胞生长的影响表现为Hormesis现象,最适刺激质量浓度为0.01 mg·L-1;低浓度硝酸铜抑制细胞生长,高浓度刺激细胞生长,0.05 mg·L-1硝酸铜处理后细胞生物量最低;基本所有浓度的硝酸铅都表现为刺激细胞生长,最适刺激质量浓度为0.5 mg·L-1。结论:重金属诱导子影响了SASC的生物量,且其作用程度和方式与氯化镧、硝酸铜、硝酸铅3种诱导子种类及浓度相关。
OBJECTIVE: To investigate the effects of abiotic elicitors and heavy metals on SASC biomass of Sorbus aucuparia (SASC). Methods: SASC was prepared by different concentrations of lanthanum chloride, copper nitrate and lead nitrate solution, and the SASC biomass was measured by electronic balance. Results: Low concentration of lanthanum chloride stimulated cell growth while high concentration inhibited cell growth. The effect on cell growth showed Hormesis phenomenon with the optimal stimulation concentration of 0.01 mg · L-1. Low concentration of copper nitrate inhibited cell growth and high The cell growth was stimulated by 0.05 mg · L-1 Cu (NO3) 2 at a concentration of 0.5 mg · L-1. Conclusion: The heavy metal inducer affects the biomass of SASC, and the degree and mode of action is related to the type and concentration of three kinds of inducer such as lanthanum chloride, copper nitrate and lead nitrate.