论文部分内容阅读
目的:探讨垂体瘤转化基因(PTTG)、血管生成内皮因子(VEGF)、p53蛋白和微血管密度(MVD)与大肠腺癌的发生发展及临床病理因素之间的关系。方法:免疫组化检测18例正常大肠黏膜组织、20例大肠腺瘤组织和44例大肠腺癌组织中PTTG、VEGF和p53表达及MVD值,分析各指标之间的相关性。结果:PTTG在正常大肠、大肠腺瘤和大肠腺癌组织中的阳性率为11.1%(2/18)、75.0%(15/20)和88.6%(39/44),VEGF阳性率为11.1%(2/18)、40.0%(8/20)和70.4%(31/44)。p53在正常大肠组织中无表达,在大肠腺瘤及大肠腺癌中的阳性表达率为50.0%(10/20)、68.2%(30/44);MVD在大肠腺癌、大肠腺瘤及正常大肠组织中的平均密度值为67±26、33±7和11±4。在大肠腺癌中PTTG和VEGF呈正相关(r=0.46,P<0.05),PTTG与p53蛋白的表达呈正相关(r=0.51,P<0.05),VEGF和p53蛋白呈正相关,r=0.32,P<0.05。结论:PTTG、VEGF和p53参与大肠腺癌的发生、发展,三者相互作用促进大肠癌新生血管的生成,促进肿瘤浸润和转移及脉管瘤栓的形成,三者可以作为大肠癌早期诊断和判断预后的有效参考指标。
Objective: To investigate the relationship between PTTG, VEGF, p53 protein and microvessel density (MVD) and the development of colorectal adenocarcinoma and the clinicopathological factors. Methods: The expressions of PTTG, VEGF, p53 and MVD in 18 cases of normal colorectal mucosa, 20 cases of colorectal adenoma and 44 cases of colorectal adenocarcinoma were detected by immunohistochemistry. The correlations among the indexes were analyzed. Results: The positive rates of PTTG in normal colorectal adenoma and colorectal adenocarcinoma were 11.1% (2/18), 75.0% (15/20) and 88.6% (39/44) respectively, and the positive rate of VEGF was 11.1% (2/18), 40.0% (8/20) and 70.4% (31/44). The positive expression rates of p53 in colorectal adenoma and colorectal adenocarcinoma were 50.0% (10/20) and 68.2% (30/44), respectively. The positive rates of MVD in colorectal adenocarcinoma, colorectal adenoma and normal The average density in the large intestine was 67 ± 26, 33 ± 7 and 11 ± 4. There was a positive correlation between the expression of PTTG and p53 protein in colorectal adenocarcinoma (r = 0.46, P <0.05), VEGF and p53 protein (r = 0.32, P <0.05. CONCLUSIONS: PTTG, VEGF and p53 are involved in the development and progression of colorectal adenocarcinoma. The three interactions promote the formation of angiogenesis in colorectal cancer and promote the infiltration and metastasis of tumor and the formation of vascular tumor thrombus. The three can be used as early diagnosis and Effective reference to determine the prognosis.