论文部分内容阅读
目的:研究建立呼吸道冠状病毒感染雏鸡的中医证候模型,观察抗戾饮对其的保护作用。方法:利用鸡传染性支气管炎病毒(infectious bronchitis virus,IBV)接种雏鸡并置于低温高湿环境中饲养,建立模型,并观察抗戾饮的保护作用。结果:IBV感染雏鸡出现高热及典型呼吸道症状,符合湿热壅滞、肺失宣降的中医证候特点,第7天死亡率为70%,病理学表现为典型的肺与支气管病变。死亡率抗戾饮高剂量组、抗戾饮中剂量组、抗戾饮低剂量组均较低,与模型组、更昔洛韦组比较,差异均有显著性意义(P<0.05)。抗戾饮高剂量组、抗戾饮中剂量组、抗戾饮低剂量组均在第4天体温明显降低、第5～6天基本恢复正常,与模型组比较,差异有非常显著性意义(P<0.01)。结论:在低温、高湿环境下利用IBV感染雏鸡,可建立较为符合中医证候特点的模型;抗戾饮对该感染模型具有改善全身症状和呼吸道症状、降低体温、改善中医证候、降低死亡率的作用。
Objective: To study the establishment of TCM syndrome models of chickens infected with respiratory coronavirus and observe the protective effect of Kangshuyin on them. METHODS: Chickens were inoculated with infectious bronchitis virus (IBV) and kept in a low temperature and high humidity environment. A model was established and the protective effect of anti-intoxication was observed. RESULTS: IBV-infected chickens showed high fever and typical respiratory symptoms, which were consistent with the features of TCM syndromes of dampness and heat stagnation and lung failure. The 7th day mortality rate was 70%. Pathological findings were typical lung and bronchial lesions. The mortality rate of high-dose anti-sputum drinking group, anti-Jinyin middle-dose group, and anti-sip drink low-dose group were all lower, compared with the model group and ganciclovir group, the difference was significant (P<0.05). The high-dose group of Kangshuyin, middle-dose group of Kangyuyin, and low-dose group of Kangshuyin all significantly decreased body temperature on the 4th day and returned to normal on the 5th to 6th day. Compared with the model group, the difference was significant ( P<0.01). Conclusion: Using IBV to infect chicks in a low temperature and high humidity environment can establish a model that is more in line with the features of TCM syndromes; Kangyingyin can improve systemic symptoms and respiratory symptoms, lower body temperature, improve TCM syndromes, and reduce death. The role of the rate.