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目的研究T淋巴细胞亚群(CD4+,CD8+)比值与多发性硬化患者发病的相关关系。方法采用间接免疫荧光染色及流式细胞仪检测28例多发性硬化(MS)活动期及缓解期外周血和脑脊液的CD4+、CD8+,MS患者活动期应用糖皮质激素治疗。结果活动期MS的外周血CD4+、CD8+细胞较对照组减少,CD4+/CD8+比值较对照组升高(P<0.01)。脑脊液中CD4+、CD4+/CD8+比值较对照组升高,CD8+细胞降低(P<0.01),且脑脊液中T淋巴细胞亚群均高于自身外周血中相应细胞(P<0.01),而且缓解期与活动期比较外周血和脑脊液CD4+/CD8+比值及脑脊液中CD4+细胞明显降低,脑脊液中CD8+明显升高(P<0.01),但外周血和脑脊液CD8+细胞仍低于对照组。结论MS患者存在T淋巴细胞辅助免疫功能的明显增强,免疫抑制功能的明显减弱;糖皮质激素对活动期MS患者有效,随着病情的缓解T淋巴细胞亚群均有不同程度的改善;部分MS患者活动期与缓解期比较,外周血和脑脊液CD4+、CD8+及CD4+/CD8+比值变化不明显,常提示容易复发。
Objective To study the relationship between T lymphocyte subsets (CD4 +, CD8 +) and the incidence of multiple sclerosis. Methods Serum levels of CD4 +, CD8 + and MS in peripheral blood and cerebrospinal fluid (CSF) of 28 patients with multiple sclerosis (MS) during active stage and remission stage were measured by indirect immunofluorescence staining and flow cytometry. Results The percentage of CD4 + and CD8 + cells in peripheral blood of MS patients was decreased and the ratio of CD4 + / CD8 + was higher than that of the control group (P <0.01). The ratio of CD4 + and CD4 + / CD8 + in cerebrospinal fluid (CSF) was higher than that in control group (P <0.01), and the level of T lymphocyte subsets in CSF was higher than that in peripheral blood (P <0.01) The ratio of CD4 + / CD8 + in peripheral blood and cerebrospinal fluid (CSF) and CD4 + cells in cerebrospinal fluid (CSF) in active stage were significantly lower than those in control group. CD8 + in cerebrospinal fluid was significantly increased (P <0.01). Conclusion MS patients with T lymphocyte immune function was significantly enhanced immune suppressive function was significantly weakened; glucocorticoid active MS patients, with the remission of T lymphocyte subsets have varying degrees of improvement; some MS Patients active and remission compared to peripheral blood and cerebrospinal fluid CD4 +, CD8 + and CD4 + / CD8 + ratio was not obvious, often suggesting that easy to relapse.