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以酿酒葡萄“赤霞珠”为研究对象,以不疏果为对照,采用顶空固相微萃取技术(HS-SPME)和气相色谱-质谱(GC-MS)联用法,研究了疏粒和疏穗处理对葡萄果实挥发性物质种类与含量的影响。结果表明:3种不同处理的“赤霞珠”葡萄果实共鉴定出48种挥发性物质,包括17种醇类、12种醛类、3种酮类、6种芳香族类、6种酯类、2种萜烯类和2种有机酸,其中,醛、酮和醇类为葡萄果实中主要的挥发性物质;在不同负载量调控下,葡萄果实中的醛、酮、醇类物质的含量均为对照高于疏粒处理,疏穗处理最低;在不同疏果处理方式下,疏粒处理的醇类香气物质种类较疏穗处理多,且1-戊醇、2-壬醇、1,10-癸二醇仅在疏粒处理中检出;对葡萄果实进行负载量调控,改变了葡萄树体的库源关系及葡萄果粒间的微域环境,从而对葡萄果实中的挥发性物质产生了一定影响,且不同的疏果方式对葡萄果实中挥发性成分的影响亦不同。
Taking grapevine Cabernet Sauvignon (Cabernet Sauvignon) as the research object and non-sparse fruit as the control, Effects of Grain and Sparse Ear Treatment on Species and Content of Volatile Matter in Grape Fruit. The results showed that 48 kinds of volatile substances including 17 kinds of alcohols, 12 kinds of aldehydes, 3 kinds of ketones, 6 kinds of aromatics and 6 kinds of aromatic species were identified in 3 different treatments of “Cabernet Sauvignon” Esters, two kinds of terpenes and two kinds of organic acids. Among them, aldehydes, ketones and alcohols were the main volatile substances in grape fruits. Under the control of different loadings, aldehydes, ketones, alcohols Were higher than the control tablets, sparse ear treatment lowest; in the different methods of thinning treatment, sparse tablets treatment of alcohol aroma substances more sparse spike treatment, and 1-pentanol, 2-nonanol, 1,10-decanediol was only detected in grain-graining treatment; the load control on the grape fruit changed the relationship between the source of the grapevine and the grapevine’s micro-domain environment, thus the volatilization in the grape fruit Sexual substances have a certain impact, and different methods of fruit thinning on the grape fruit volatile components are also different.