论文部分内容阅读
目的 :检测厌食患儿免疫功能 ,探讨外周血淋巴细胞凋亡 (PCD)过程。方法 :检测 5 8例厌食患儿外周血凋亡淋巴细胞和免疫功能 ,并与 2 8例健康儿童对照。T淋巴细胞亚群采用间接免疫荧光法 ,免疫球蛋白采用免疫比浊法 ,细胞凋亡用口丫 啶橙 溴化乙锭染色法。结果 :该组患者免疫功能低下 ,主要表现为IgG、CD+4、CD+4/CD+8明显降低 ,IgM、CD+8明显升高。PCD率明显升高。结论 :PCD过度是该组患者免疫功能低下的一个重要原因。
Objective: To detect the immune function in children with anorexia and explore the process of peripheral blood lymphocyte apoptosis (PCD). Methods: Peripheral blood apoptotic lymphocytes and immune function in 58 cases of anorexia children were detected and compared with 28 healthy children. T lymphocyte subsets by indirect immunofluorescence, immunoglobulin by immunoturbidimetry, apoptosis with oral acridine orange ethidium bromide staining. Results: The immunocompromised patients in this group showed low levels of IgG, CD 4, CD 4 / CD 8 and significantly increased IgM and CD 8. PCD rate increased significantly. Conclusions: PCD is an important reason for the immunocompromised patients in this group.