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目的通过观察川崎病(KD)患儿血清粒细胞集落刺激因子(GCSF)、白细胞介素8(IL8)水平的变化及外周血中性粒细胞计数(PBN)的改变,探讨预测KD患儿冠状动脉损害(CAL)的新指标。方法采用双抗体酶联免疫吸附法(ELISA)检测42例KD患儿、25例急性感染患儿及28例健康儿童血清GCSF、IL8水平,同时采用全自动血球分析仪检测PBN,观察上述指标在三组间的差别,并观察其在KD并发CAL与否者(CAL亚组、nonCAL亚组)的区别。结果KD患儿血清GCSF、IL8及PBN均明显高于感染对照组(P<0.01)及健康对照组(P<0.01)。GCSF在CAL亚组显著高于nonCAL亚组(P<0.05),而IL8和PBN在KD组的两亚组间差异无统计学意义(P>0.05)。结论GCSF、IL8及PBN均参与KD的发生,其中GCSF可能起关键性作用,可望作为预测CAL的指标之一。
Objective To observe the changes of serum granulocyte colony stimulating factor (GCSF), interleukin 8 (IL8) levels and peripheral blood neutrophil count (PBN) in children with Kawasaki disease (KD) A new indicator of arterial damage (CAL). Methods The serum levels of GCSF and IL8 in 42 children with KD, 25 children with acute infection and 28 healthy children were detected by double antibody enzyme-linked immunosorbent assay (ELISA). Meanwhile, PBN was detected by automatic hematology analyzer Differences among the three groups were observed and their differences between KD-matched CALs and CALs (CAL subgroup, non-CAL subgroup) were observed. Results The serum levels of GCSF, IL8 and PBN in children with KD were significantly higher than those in infected control group (P <0.01) and healthy controls (P <0.01). GCSF was significantly higher in the CAL subgroup than in the nonCAL subgroup (P <0.05), while there was no significant difference between the two subgroups in the KD subgroup (P> 0.05). Conclusions Both GCSF, IL8 and PBN are involved in the pathogenesis of KD. GCSF may play a key role in predicting CAL.