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目的探讨幽门螺杆菌(Helicobacter pylori,Hp)感染对胃癌侵袭和淋巴结转移的影响以及金属基质蛋白酶-2(matrix metalloproteinase-2,MMP-2)、金属基质蛋白酶组织抑制因子-2(tissue inhibitor of matrix metalloproteinase,TIMP-2)在胃癌组织中表达。方法 78例胃癌患者分为Hp感染组(45例)和非Hp感染组(33例),使用快速尿素酶试验和改良Giemsa染色检测Hp感染,使用免疫组织化学染色法检测胃癌组织中MMP-2和TIMP-2的表达。结果与非Hp感染组相比,Hp感染组发生浆膜侵犯、淋巴结转移的比例和MMP-2阳性率均增高,而T1期比例和TIMP-2阳性率均下降(P<0.05)。与无浆膜侵犯或无淋巴结转移的患者相比,有浆膜侵犯或有淋巴结转移患者MMP-2阳性率增高,TIMP-2阳性率下降(P<0.05)。结论 Hp感染能使胃癌浆膜侵袭和淋巴结转移发生率增加,其机制可能与Hp感染上调MMP-2和下调TIMP-2水平有关。
Objective To investigate the effects of Helicobacter pylori (Hp) infection on the invasion and lymph node metastasis of gastric carcinoma and the effects of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix Metalloproteinase, TIMP-2) in gastric cancer. Methods 78 cases of gastric cancer were divided into Hp infection group (n = 45) and non-Hp infection group (33 cases). Hp infection was detected by rapid urease test and modified Giemsa staining. The expression of MMP-2 in gastric cancer tissue was detected by immunohistochemical staining And TIMP-2 expression. Results Compared with non-Hp infection group, serosal invasion, lymph node metastasis and MMP-2 positive rate were all increased in Hp infection group, while the proportion of T1 stage and TIMP-2 positive rate were decreased (P <0.05). Compared with patients without invasion or lymph node metastasis, the positive rate of MMP-2 and TIMP-2 positive rate in patients with serosa invasion or lymph node metastasis were significantly decreased (P <0.05). Conclusions Hp infection can increase the incidence of serosa invasion and lymph node metastasis in gastric cancer, which may be related to up-regulation of MMP-2 and down-regulation of TIMP-2 by Hp infection.