光散射信号在药物颗粒分析检测中具有广泛应用.本文将其应用于中药中汞残留量测定.研究发现,-氨基酸与水合茚三酮(Ninhydrin)反应形成的罗曼紫(Ruhemann’s purple,RP)复合试剂在pH5.5醋酸-醋酸钠缓冲溶液中能和汞(Hg2+)形成配合物,溶液由蓝紫色变为粉红色,对应的光散射信号随着Hg2+浓度增大而增强.据此,本文在评价该复合试剂能应用于分析测定的基础上,建立了Hg2+的共振光散射测定法.340nm处光散射增强强度和8.0×107~6.0×105mol/L的Hg2+呈良好的线性关系,方法检出限(3)为6.2×108mol/L.用于养血安神片、云南白药粉末和逍遥丸三种中药制剂中残留汞的加标回收测定,回收率在95.7%~106.5%范围内,相对标准偏差小于3.94%. The light scattering signal is widely used in the analysis of drug particles.This paper is applied to the determination of mercury in traditional Chinese medicine.It is found that Ruhemann’s purple (RP) complex formed by the reaction of amino acids with Ninhydrin The reagent can form a complex with mercury (Hg2 +) in pH5.5 acetate-sodium acetate buffer solution, the solution changes from blue-purple to pink, and the corresponding light scattering signal increases with the increase of Hg2 + concentration.Accordingly, The complex reagent can be applied to the analysis and determination based on the established Hg2 + resonance light scattering determination of light scattering intensity at 340nm and 8.0 × 107 ~ 6.0 × 105mol / L of Hg2 + showed a good linear relationship, the method was detected The limit (3) was 6.2 × 108mol / L. The recoveries of spiked levels of residual mercury in three traditional Chinese medicine preparations, namely nourishing blood and relieving aphrodisiac tablets, Yunnanbaiyao powder and Xiaoyao pill, were in the range of 95.7% -106.5% The deviation is less than 3.94%.