论文部分内容阅读
目的:观察三七总皂苷(panax notoginseng saponins,PNS)对拟缺血损伤脑微血管内皮细胞胞浆型磷脂酶A2IVA(phospholipase A2 group IVA,PLA2G4A)表达及其功能的影响。方法:培养大鼠原代脑微血管内皮细胞,氧糖剥夺法制备拟缺血模型,用三七总皂苷及PLA2G4A抑制剂进行干预,通过RT-PCR和Western blotting法检测各组PLA2G4A基因及蛋白表达水平,ELISA法检测各组花生四烯酸(arachidonic acid,AA)、20-羟二十烷四烯酸(20-hydroxyeicosatetraenoic acid,20-HETE)、血栓素A2(thromboxanea2,TXA2)/前列环素(prostaglandin I2,PGI2)表达水平。结果:三七总皂苷可显著下调PLA2G4A的基因和蛋白表达,对AA、20-HETE、TXA2/PGI2也有下调作用。结论:三七总皂苷可以下调拟缺血损伤脑微血管内皮细胞PLA2G4A及其下游产物的表达,这可能是三七总皂苷在脑缺血损伤中发挥化瘀通络作用的机制之一。
Objective: To observe the effect of panax notoginseng saponins (PNS) on the expression of phospholipase A2 group IVA (PLA2G4A) and its function in ischemic injured brain microvascular endothelial cells. Methods: Primary cerebral microvascular endothelial cells were cultured in vitro. The ischemic model was prepared by oxygen-glucose deprivation method. Panax notoginseng saponins and PLA2G4A inhibitor were used for intervention. The expression of PLA2G4A gene and protein in each group was detected by RT-PCR and Western blotting The levels of arachidonic acid (AA), 20-hydroxyeicosatetraenoic acid (20-HETE), thromboxane A2 (TXA2) / prostacyclin (prostaglandin I2, PGI2) expression levels. Results: Panax notoginseng could significantly down-regulate the gene and protein expression of PLA2G4A, and also down-regulate the expression of AA, 20-HETE and TXA2 / PGI2. Conclusion: The total saponin of Panax notoginseng can down-regulate the expression of PLA2G4A and its downstream products in ischemic injury-induced brain microvascular endothelial cells, which may be one of the mechanisms that Panax Notoginseng Saponins can play the role of clearing blood stasis and collaterals in cerebral ischemia injury.