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目的:探讨有氧运动和饮食干预对胰岛素抵抗(IR)小鼠骨骼肌球形脂联素(G-Adiponectin)及腺苷酸活化蛋白激酶(AMPK)的影响,为运动和饮食辅助防治IR的研究提供理论依据。方法:选用雄性C57BL/6小鼠30只,随机分为正常饮食和IR模型组,后者喂养8周高脂饲料后建立胰岛素抵抗(IR)模型,再将IR模型组分为高脂饮食安静组(HC)、高脂饮食运动组(HE)、正常饮食安静组(HNC)和正常饮食运动组(HNE)。运动组进行6周跑台训练,高脂饮食组继续6周的高脂饮食。实验8周后,采用ELISA法检测小鼠血清空腹胰岛素水平及以口服糖耐量试验(OGTT)检测葡萄糖耐量。14周后,采用NorthernBlot检测腓肠肌AMPKmRNA表达,WesternBlot和免疫荧光染色检测腓肠肌G-Adiponectin、AMPK和pAMPK-Thr172蛋白表达。采用酶法测定小鼠血清甘油三酯(TG)和胆固醇(TC)含量。结果:HE和HNE组骨骼肌细胞AMPKmRNA和蛋白以及pAMPK-Thr172蛋白分别较HC及HNC组表达显著增高;HNE组G-Adiponectin显著高于HNC组;HNC组AMPK蛋白和mRNA表达均显著高于HC组;各组免疫荧光检测结果与WesternBlot结果一致。结论:有氧运动和饮食干预均能有效增强Adiponectin-AMPK信号通路,改善机体的脂质代谢紊乱,从而产生防治IR的效果。
Objective: To investigate the effects of aerobic exercise and dietary intervention on the expression of G-Adiponectin and AMPK in skeletal muscle of insulin-resistant (IR) mice and to study the effects of exercises and dietary supplementation on the prevention and treatment of IR Provide a theoretical basis. Methods: Thirty male C57BL / 6 mice were randomly divided into normal diet and IR model group. The latter group were fed with high fat diet for 8 weeks to establish insulin resistance (IR) model. The IR model group was divided into high fat diet (HC), high fat diet group (HE), normal diet quiet group (HNC) and normal diet group (HNE). Exercise group treadmill training for 6 weeks, high-fat diet group to continue six weeks of high-fat diet. Eight weeks after the experiment, serum insulin levels were measured by ELISA and glucose tolerance was measured by oral glucose tolerance test (OGTT). The expression of AMPK mRNA in gastrocnemius muscle was detected by NorthernBlot after 14 weeks. The expression of G-Adiponectin, AMPK and pAMPK-Thr172 protein in gastrocnemius muscle was detected by Western Blot and immunofluorescence staining. Serum triglyceride (TG) and cholesterol (TC) levels were determined by enzymatic method. Results: The expressions of AMPK mRNA and protein and pAMPK-Thr172 protein in HE and HNE group were significantly higher than those in HC and HNC group respectively. The G-Adiponectin in HNE group was significantly higher than that in HNC group. The expression of AMPK protein and mRNA in HNC group was significantly higher than that of HC group Group; the results of immunofluorescence in each group were consistent with WesternBlot results. Conclusion: Both aerobic exercise and diet intervention can effectively enhance the Adiponectin-AMPK signaling pathway, improve the body’s lipid metabolism disorders, and thus have the effect of preventing and treating IR.