论文部分内容阅读
目的探讨IFN-γ、重组TGF-β1 RII蛋白对小鼠日本血吸虫病肝纤维化的影响。方法昆明小鼠(6~8周龄,体重18~25g,雌雄各半),随机分成6组(6只/组),正常对照组除外,其余30只通过腹部皮肤感染日本血吸虫尾蚴18+2条/鼠,六周后成功建立小鼠血吸虫性肝纤维化模型,具体治疗分组:①正常对照组;②模型对照组;③吡喹酮(PQT)治疗组;④PQZ+IFN-γ治疗组;⑤PQT+rTGF-β1RII蛋白治疗组;⑥PQT+IFN-γ+rTGF-β1 RII蛋白治疗组。第九周末处死小鼠,HE染色法对肝组织进行病理学检查;TBA法测肝匀浆MDA含量。结果 IFN-γ、rTGF-β1RII蛋白治疗显著降低肝纤维化指数,抑制日本血吸虫诱导的肝MDA水平的升高。结论日本血吸虫病化学药物治疗中,以PQT为基础配合rTGF-β1RⅡ蛋白、IFN-γ的联合疗法策略优于PQT单独用药策略;rTGF-β1RⅡ蛋白、IFN-γ的抗纤维化效应可能与其抗氧化功能有关。
Objective To investigate the effects of IFN-γ and recombinant TGF-β1 RII on hepatic fibrosis in mice with Schistosoma japonicum. Methods Kunming mice (6-8 weeks old, body weight 18-25g, male and female) were randomly divided into 6 groups (6 rats / group) except the normal control group. The remaining 30 mice were infected with 18 + 2 The mice were randomly divided into 4 groups: control group, model control group, praziquantel (PQT) treatment group, PQZ + IFN-γ treatment group, ⑤ PQT + rTGF-β1RII protein treatment group; ⑥ PQT + IFN-γ + rTGF-β1 RII protein treatment group. Mice were sacrificed on the 9th weekend. HE staining was used to detect the pathological changes of liver tissue. The content of MDA in liver homogenate was measured by TBA method. Results Treatment with IFN-γ and rTGF-β1RII significantly reduced the hepatic fibrosis index and inhibited the increase of liver MDA level induced by Schistosoma japonicum. Conclusion The combination therapy of rTGF-β1RⅡ and IFN-γ based on PQT is superior to PQT alone in the treatment of schistosomiasis japonica. The anti-fibrotic effect of rTGF-β1RⅡ and IFN-γ may be related to its anti-oxidation Function related.