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目的：探讨内毒素对大鼠肝、肠组织内皮素受体（ＥＴＲ）活性变化及内皮素Ａ型受体ｍＲＮＡ转录表达的影响。方法：选用Ｗｉｓｔａｒ大鼠３０只，分为对照组、内毒素组。自左股静脉给予１０ｍｇ／ｋｇ内毒素，观察了伤后３、６、１２、２４ｈ４个时相点肝、肠组织ＥＴＲ活性和内皮素Ａ型受体ｍＲＮＡ相对含量。结果：肝、肠组织ＥＴＲ的解离常数无明显变化，最大结合数伤后３ｈ明显降低，持续２４ｈ。内皮素Ａ型受体ｍＲＮＡ相对含量均高于对照组。结论：内毒素能使肝、肠细胞膜表面ＥＴＲ的最大结合数目降低；并在转录和翻译水平使内皮素Ａ型受体合成增加。
Objective: To investigate the effect of endotoxin on the activity of endothelin receptor (ETR) and the transcription of endothelin A receptor mRNA in liver and intestine of rats. Methods: Thirty Wistar rats were randomly divided into control group and endotoxin group. 10 mg / kg endotoxin was given from the left femoral vein and ETR activity and relative content of endothelin A receptor mRNA in the liver and intestine tissues at 3, 6, 12 and 24 h after injury were observed. Results: The dissociation constants of ETR in liver and intestine tissues showed no significant change. The maximum binding number of ETR decreased significantly after 3 hours and lasted for 24 hours. Endothelin A receptor mRNA relative content were higher than the control group. Conclusion: Endotoxin can reduce the maximum number of ETR on the surface of liver and intestinal cell membrane, and increase the synthesis of endothelin A receptor at transcription and translation level.