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目的筛选出消毒实验中评价消毒因子对病毒灭活效果的指示噬菌体,研究噬菌T4、φχ174D和f2对二氯异氰尿酸钠(NaDCC)消毒剂的抵抗力。方法消毒剂对噬菌体的灭活效果采用悬液定量灭活实验、中和剂鉴定实验,噬菌体的检测采用双层琼脂法。结果(1)有效氯浓度为150mg/L的NaDCC溶液与噬菌体T4作用40min,或有效氯浓度为300mg/L作用5min,即可达到消毒水平[对噬菌体T4的灭活对数值或噬菌体T4的对数减少值(log10No-log10Nt)≥4.00log10]。(2)有效氯浓度为300mg/L的NaDCC溶液与噬菌体φχ174D作用5min,或有效氯浓度为400mg/L作用3min达到消毒。(3)有效氯浓度为2000mg/L的NaDCC溶液与噬菌体f2作用20min,或有效氯浓度为4000mg/L作用5min达到消毒。结论上述3种噬菌体对NaDCC的抵抗力为噬菌体f2>噬菌体T4>噬菌体φχ174D。
Objective To screen out phage displayed by phagosomes in disinfection experiment to evaluate the inactivation effect of disinfectant on virus and study the resistance of phage T4, φχ174D and f2 to sodium dichloroisocyanurate (NaDCC) disinfectant. Methods Inactivation of bacteriophage by disinfectant suspension quantitative inactivation experiments, neutralizing agent identification experiments, phage detection using double agar. Results (1) The concentration of 150mg / L of NaDCC solution with phage T4 for 40min, or the effective chlorine concentration of 300mg / L for 5min, can reach the level of disinfection [phage T4 inactivation logarithm phage T4 or Number reduction (log10No-log10Nt) ≥4.00log10]. (2) The effective chlorine concentration of 300mg / L NaDCC solution and phage φχ174D role 5min, or the effective chlorine concentration of 400mg / L for 3min to achieve disinfection. (3) The effective chlorine concentration 2000mg / L of NaDCC solution and phage f2 role 20min, or effective chlorine concentration of 4000mg / L role in 5min to disinfection. Conclusion The resistance of these three phage to NaDCC is phage f2> phage T4> phage φχ174D.