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采用qPCR方法检测黄独4℃低温离体保存微型块茎几种酶的活性.研究表明:在黄独微型块茎的4℃低温离体保存中,谷胱甘肽过氧化物酶和过氧化物酶基因上调,表达量升高,其他酶基因(过氧化氢酶,抗坏血酸过氧化物酶,超氧化物歧化酶l (Cu-Zn),谷胱甘肽还原酶,多聚半乳糖醛酸酶,谷胱甘肽硫转移酶,查尔酮合酶,高柠檬酸合酶,超氧化物歧化酶1 (Cu-Zn)和蔗糖合成酶均下调),表达量下降,而转录组的结果表明多聚半乳糖醛酸酶、谷胱甘肽硫转移酶、查尔酮合酶和高柠檬酸合酶基因上调,表达量升高,其他酶基因(过氧化氢酶,抗坏血酸过氧化物酶,超氧化物歧化酶1 (Cu-Zn),谷胱甘肽还原酶,超氧化物歧化酶1(Cu-Mn),谷胱甘肽过氧化物酶,过氧化物酶和蔗糖合成酶)均下调,表达量下降.这个结果表明,在qPCR检测和转录组结果中,绝大部分酶基因的表达趋势相一致,一小部分基因出现相反表达趋势,可能是由于产物的非特异性扩增造成的.本试验结果可为黄独微型块茎的低温离体保存提供理论依据.“,”The activity of some enzymes of Dioscorea bulbifera L.microtuber conserved in vitro at 4℃ low temperature was detected by qPCR.The research showed that:In the conservation in vitro ofDioscorea bulbifera L.microtuber at 4℃ low temperature,glutathione peroxidase gene and peroxidase gene were up-regulated and their expression increased,other genes (catalase,ascorbate peroxidase,superoxide dismutase 1 (Cu-Zn),glutathione reductase,polygalacturonase,glutathione S-transferase,chalcone synthase,citrate synthase and superoxide dismutasel (Cu-Zn) and sucrose synthase) were down-regulated and the expression decreased,while the transcriptome results show that polygalacturonase gene,glutathione S-transferase gene,chalcone synthase gene and high citrate synthase gene were up-regulated and their expression increased,other gens (catalase,ascorbate peroxidase,superoxide dismutase 1 (Cu-Zn),glutathione reductase,superoxide dismutase 1 (Cu-Mn),glutathione peroxidase,catalase and sucrose synthase) were down-regulated and the expression decreased.TheresultsshowedthatintheqPCRdetectionand the transcriptome results,the expression trend of most enzyme genes was consistent,only gene expression trend of a few genes was opposite,which may be due to nonspecific amplification of products.The test results can beusedto provide a theoretical basis for the conservation in vitro ofDioscorea bulbifera L.microtuber at 4℃ low temperature.