Momordica grosvenori is a valuable edible plant with medicinal purposes,and it is widely used in medicated diets and traditional Chinese medicine in Asia.Mogroside V (MV),the main bioactive component from M.grosvenori,is commonly used as a natural sweetener.M.grosvenori extracts have been reported to exert potent anti-inflammatory property,however the underlying molecular mechanism still remains unknown.In the present study,the biological effect of MV in inflammation was investigated in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.The ELISA and weste blot analysis results showed that MV significantly inhibited LPS-induced prostaglandin E2 (PGE2) production and cyclooxygenase-2 (COX-2) expression.MV markedly decreased the phosphorylation of IκB-α,increased IκB-α,and reduced nuclear p-65 and C/EBPδ.Furthermore,MV attenuated LPS-induced phosphorylation of MAPKs and AKT1,and only the phosphorylation status of AKT1 was found to be consistent with the expression trend of COX-2.Moreover,MV reduced ROS level and restored overexpressed HO-1 and AP-1 to basal level,which can be markedly reversed by AKT1 inhibitor LY294002.These results revealed that AKT1 plays a key role in LPS-induced COX-2 expression,and acts as a mediator to keep the redox balance in LPS-stimulated RAW264.7 cells.MV exerts anti-inflammatory property by blocking AKT1-mediated NF-κB and C/EBPδ activation,ROS generation and AP-1/HO-1 expression.Therefore,the present study indicated that MV has a significant chemopreventive effect on the inflammatory lesions and suggested that AKT1 is a potential specific target of MV for relieving inflammation.