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目的研究阿托伐他汀对体外大鼠主动脉平滑肌细胞钙化(VSMC)的作用。方法采用组织块培养法体外培养大鼠主动脉平滑肌细胞。细胞分5组,即正常组(常规细胞培养液)、钙化组(加入10 mmol／Lβ-磷酸甘油、1×10-7mol／L胰岛素及50μg／L维生素C钙化培养基)和阿托伐他汀(1μmol／L、5μmol／L和10μmol／L)组,后者在诱导血管平滑肌细胞钙化之前,分别给予阿托伐他汀1μmol／L、5μmol/L、10μmol／L预处理24 h后,再加入钙化培养基诱导细胞钙化,连续培养14 d。细胞爬片茜素红S染色观察VSMC钙化;比色法测定细胞Ca2+浓度和细胞蛋白质含量,两者之比作为细胞钙沉积含量;比色法测定细胞ALP活力;MTT法检测细胞增殖。结果阿托伐他汀各组钙结节计数减少,细胞钙沉积含量减少,ALP活力和细胞增殖均降低,并呈剂量依赖性。结论阿托伐他汀对大鼠主动脉平滑肌细胞体外钙化有抑制作用。
Objective To investigate the effect of atorvastatin on the in vitro aortic smooth muscle cell calcification (VSMC). Methods Cultured rat aortic smooth muscle cells were cultured in tissue culture. The cells were divided into 5 groups: normal group (conventional cell culture medium), calcification group (10 mmol / L β-phosphoglycerol, 1 × 10-7 mol / L insulin and 50 μg / L vitamin C calcification medium) and atorvastatin (1μmol / L, 5μmol / L and 10μmol / L), which were given atorvastatin 1μmol / L, 5μmol / L and 10μmol / L for 24 h before inducing vascular smooth muscle cell calcification. Calcification medium induced cell calcification, continuous culture for 14 days. The cells were stained with alizarin red S to observe the calcification of VSMC. The cell Ca2 + concentration and cellular protein content were determined by colorimetric method. The ratio of the two was used as the calcium deposition. The ALP activity was measured by colorimetric assay. Cell proliferation was detected by MTT assay. Results Atorvastatin reduced the number of calcium nodules, reduced the content of calcium in cells, ALP activity and cell proliferation were reduced in a dose - dependent manner. Conclusions Atorvastatin can inhibit the in vitro calcification of aortic smooth muscle cells in rats.