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分析了枯萎病菌接种后棉苗组织内儿茶素含量的变化以及儿茶素对枯萎病菌及其多聚半乳糖醛酸酶活性的影响。健康棉苗组织中儿茶素含量随着棉苗的生长而增加。枯萎病菌接种后,病苗组织中儿茶素含量显著高于健康对照,而且抗病品种棉苗儿茶素含量的增加明显高于感病品种。经氟乐灵预处理的棉苗接种枯萎病菌后,儿茶素含量增加更快,增幅更大。说明儿茶素是品种抗性和氟乐灵诱发棉苗对枯萎病诱导抗性的机理之一。儿茶素可抑制枯萎病菌的菌丝生长、产孢及孢子萌发,对培养液中病菌的多聚半乳糖醛酸酶(PG)有抑制作用。儿茶素化学制品及棉苗组织提取液经TLC分离后获得的含儿茶素组分提取液对初步纯化的PG活性有明显的抑制作用。这些结果表明:儿茶素可能通过抑制病菌的菌丝生长、产孢以及其PG活性而在棉花对枯萎病的抗病性中起到作用。
The changes of catechin content in cotton seedling tissues after inoculation of Fusarium oxysporum and the effects of catechins on Fusarium oxysporum and polygalacturonase activity were analyzed. Cotyledon content in healthy cotton seedling tissues increases with the growth of cotton seedlings. After inoculation with Fusarium oxysporum, the content of catechins in the seedling tissues was significantly higher than that in healthy controls, and the increase of catechin content in resistant varieties was obviously higher than that in susceptible varieties. The cotton seedlings treated with trifluralin showed a faster and larger increase in the content of catechins after inoculation with Fusarium oxysporum f. Catechin is one of the mechanisms of variety resistance and trifluralin-induced resistance to Fusarium wilt. Catechin can inhibit the growth of Fusarium oxysporum mycelium, sporulation and spore germination, polygalacturonase (PG) inhibition of bacteria in the culture medium. Catechin chemical products and tissue extracts of cotton seed extract obtained by TLC containing catechin extract of the initial purified PG activity was significantly inhibited. These results indicate that catechins may play a role in the resistance of cotton to Fusarium wilt by inhibiting mycelial growth, sporulation and PG activity.