目的了解成都地区2010年7～12月之间人杯状病毒感染性腹泻的情况。方法采集成都市2家哨点医院2010年7～12月之间腹泻病人的大便标本501份,采用RT-PCR方法,对501份大便标本同时进行诺如病毒、札如病毒核酸检测。结果 501份标本中检出阳性122份,核酸阳性率为24.35%(122/501),其中诺如病毒GⅠ型1份,占阳性标本的0.82%(1/122);诺如病毒GⅡ型100份,占阳性标本的81.97%(100/122);札如病毒18份,占阳性标本的14.75%(18/122);诺如病毒GⅡ型和札如病毒共同感染3份,占阳性标本的2.46%(3/122)。5岁以下儿童患者标本病毒核酸阳性检出率为24.3%(109/449),其中诺如病毒GⅡ型的检出率最高;5～18岁患者标本病毒核酸阳性检出率为14.29%(1/7),标本检验结果为诺如病毒GⅡ型;18岁以上患者标本阳性检出率为26.67%(12/45),其中诺如病毒GⅡ型检出率最高。9、10、11 3个月病毒核酸检出率较高,其中9月份病毒核酸检出率最高为36.51%(23/63)。结论成都地区人杯状病毒感染性腹泻的病原体以诺如病毒GⅡ型为主,主要在秋冬季节高发,且婴幼儿和成人同样容易感染。 Objective To understand the case of human calicivirus-associated diarrhea in Chengdu area from July to December in 2010. Methods 501 stool samples of diarrhea patients in two sentinel hospitals in Chengdu from July to December 2010 were collected. 501 stool samples were simultaneously detected for Norovirus and Sappan virus by RT-PCR. Results A total of 122 positive samples were detected in 501 samples, with a positive rate of 24.35% (122/501). Among them, 1 was GV genotype of Norovirus, accounting for 0.82% (1/122) of positive samples; norovirus G Ⅱ 100 , Accounting for 81.97% (100/122) of the positive samples; 18 copies of the Zha-Ru virus, accounting for 14.75% (18/122) of the positive samples; 3 norovirus co-infections with the Sappus virus 2.46% (3/122). The detection rate of nucleic acid positive samples was 24.3% (109/449) in children under 5 years of age, of which the detection rate of Norovirus type G Ⅱ was the highest. The detection rate of nucleic acid of specimens of patients aged 5-18 years was 14.29% (1 / 7). The test result of the specimen was Norovirus GⅡ. The positive rate of the specimens was 26.67% (12/45) in patients over 18 years of age, and the detection rate of Norovirus was the highest. The detection rate of virus nucleic acid was high at 9, 10, and 11 months, of which the highest detection rate of virus nucleic acid in September was 36.51% (23/63). Conclusions The pathogen of human calicivirus-associated diarrhea in Chengdu is predominantly Norovirus type GⅡ, mainly in autumn and winter. Infants and young children are equally susceptible to infection.