论文部分内容阅读
东方铃蟾鲜皮的甲醇提取液,可引起大鼠离体平滑肌的收缩。此液经碱性氧化铝柱层析分离,其80%乙醇洗脱的 C 组分显示生物活性。C 组分再经葡聚糖凝胶 G-15柱分离,其活性较强的早期洗脱组分 C_(_12),可被糜蛋白酶水解灭活,但其活性并不被5-HT 拮抗剂赛庚啶[2×10~(-6)mol/L]完全拮抗。继用反相高效液相色谱(RP-HPLC)分析,见分离出的 C_(_12_h)峰与铃蟾肽~*(Bombesin,BBS)的出峰时间相同,且具相同的氨基酸组成。上述实验结果提示,C_(_12_h)肽可能就是铃蟾肽。
Oriental beetle fresh skin methanol extract, can cause rat isolated smooth muscle contraction. This liquid was separated by basic alumina column chromatography and its C component eluted with 80% ethanol showed biological activity. C component was separated by G-15 Sephadex G-15 column, its active early component C _ (_ 12) was hydrolyzed by chymotrypsin, but its activity was not inhibited by 5-HT antagonist Cyproheptadine [2 × 10 -6 mol / L] completely antagonized. After analysis by reversed-phase high performance liquid chromatography (RP-HPLC), the peak of C_ (12_h) was found to be the same as that of bombesin ~ (Bombesin, BBS) and had the same amino acid composition. The above experimental results suggest that C _ (_ 12_h) peptide may be bombesin.