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纳米乳具有良好的促进药物透皮吸收作用,但对于完整的纳米乳能否透入皮肤仍然知之有限。本研究通过聚集淬灭荧光探针(P4)标记实现纳米乳的整体识别,以香豆素6(C6)模拟载带药物,探索纳米乳在完整大鼠皮肤和经微针处理大鼠皮肤中的经皮转运情况。通过单因素试验优化,分别制备了粒径为80和500 nm的纳米乳。完整皮肤给药后4 h,2种粒径纳米乳均不能完整、高效地进入皮肤,但小粒径纳米乳能够在毛囊聚集,并向周围皮肤组织扩散C6。微针处理显著提高了纳米乳的经皮转运,小粒径纳米乳比大粒径纳米乳更易于进入皮肤深部,并向更深处皮肤组织扩散C6。
Nanoemulsion has a good effect of promoting drug transdermal absorption, but it still has limited knowledge of whether the intact nanoemulsion penetrates the skin. In this study, the whole recognition of nanoemulsion was achieved by aggregating and quenching the fluorescent probe (P4), and coumarin 6 (C6) was used to mimic the carrier drug to explore the effect of nanoemulsion on intact rat’s skin and microneedle treated rat’s skin Transdermal delivery. Through the optimization of single factor experiments, nanoemulsion with particle size of 80 and 500 nm were prepared respectively. At 4 h after complete dermal administration, the two types of nanoemulsions failed to penetrate into the skin completely and efficiently, but small-sized nanoemulsion could accumulate in the hair follicles and spread to the surrounding skin tissue C6. Microneedle treatment significantly enhances the transdermal delivery of nanoemulsion. The smaller particle size nanoemulsion is easier to penetrate deeper into the skin than the larger particle size nanoemulsion and diffuses to deeper skin tissue C6.